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KMID : 0903519930360010045
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1993 Volume.36 No. 1 p.45 ~ p.50
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Isolation of psychrotrophic microorganism producing soymilk - clotting enzyme from marine fish
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Abstract
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A psychrotrophic microorganism isolated from Alaska pollack (Theragra chalcoramma) produced soymilk-clotting enzymes) with relatively low proteolytic activity. The isolate No. 268 was tentatively identified as Pseudomonas sp. Soymilk-clotting activity of the crude enzyme solution was observed at temperatures ranging from 20 to 60℃ and the optimum temperature was 40℃. When the crude enzyme solution was preincubated for 30 minutes, the clotting activity was stable at temperatures up to 30℃ and 75℃ of the activity was retained at 40℃. The clotting activity was decreased as the pH of soymilk was increased from 5.8 to 7.3.
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